Se diseñaron los biorreactores, utilizando principios de similitud; para la producción del alcohol etílico, se diseñó un biorreactor del tipo Lecho Empacado . Inmovilización de levaduras en residuos lignocelulósicos para la producción de etanol en biorreactor de lecho empacado. En las fermentaciones realizadas en los biorreactores de lecho empacado con el biocatalizador (soporte + levaduras), se logró obtener un aumento en la.
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Modelado de reactores de lecho empacado en COMSOL®
Reunir un gran volumen al menos 10 L de agua de un cuerpo de agua que contiene algas, tales como una corriente o estanque. The baseline immobilization was performed by following the yeast immobilization protocol.
The experimental design analysis using Design Expert software allowed us to establish that there is no significant statistical difference between treatments. The results obtained have allowed us to establish the potential this lignocellulosic waste has as carriers for cell immobilization for the continuous production of ethanol, and the possibility of using glucose syrup as an alternative substrate.
English pdf Article in xml format Article references How to cite this article Automatic translation Send this article by e-mail. Apriete el tubo para asegurar que todo el aire ha sido expulsado. Recibido el 5 de abril de Practical Insight To Tools Applications. We studied various techniques spectrophotometry, protein quantification and dry weight ; the better results were obtained with the dry weight technique, which was modified to allow direct quantification of biomass immobilized on the carrier.
Fill out the form below to receive a free trial or learn more about access:. At the output of the reactor we obtained concentrations from In addition, the results have high deviations.
Esquema de un biorreactor de alta densidad HDBR no t escala o.
Conclusions In this study we developed a protocol for quantification of immobilized biomass on lignocellulosic carriers and we obtained reproducible results. The immobilization matrixes more frequently used have been polymeric gels like alginate, where the cells are immobilized for entrapment into the gel.
Un biorreactor Novela de alta densidad de cultivo de diversas comunidades microbianas
Ajuste la bomba a 25 revoluciones min -1, estableciendo un caudal de 1,5 ml min The results have established the implementation potential of ethanol production by continuous fermentation with immobilized cells in waste lignocellulosic materials. Subsequently, the bioreactor was filled with fresh culture medium and allowed to operate with recirculation for 12 hours .
A Cole Parmer peristaltic pump model was used, operating at a flow of 0. As shown in the results of table 3, it is the cane bagasse the material on which the biggest quantity of biomass was immobilized.
However, these values are higher than the ones obtained in biorraector fermentation with glucose. We kindly thank the Biotechnology and Biotransformation Groups of the Universidad de Antioquia for their support.
We have also established that the glucose syrup obtained from the enzymatic hydrolysis of cassava flour can be used as an alternative substrate in alcoholic fermentations. The same process was implemented with cell-free material to be used as blank. The concentration of biomass was determined by optic density at nm using a curve of dry weight. An important result in this experimentation was the confirmation of reproducibility of operational conditions of fermentations and stability of packed bed system during operation time.
Natural immobilisation of microorganisms for continuous etanol production, Enzyme and Microbial technology, 40, pp. The results of the fermentations carried out with glucose syrup obtained from cassava flour are shown in Figs. In this study we developed a protocol for quantification of immobilized biomass on lignocellulosic carriers and we obtained reproducible results.
Used as reactor vessel R. Journal ofBioscience and Bioengineering. From Whole Cell Immobilization: Mida la biomasa dentro de la muestra concentrada.
Revista Facultad de Ingeniería
The proposed hypothesis is biorreaactor these materials are appropriate matrices for the immobilization of yeast cells, which allow high cellular activity, lasting stability and they enable continuous operation for the production of ethanol. Immobilization matrices Used as immobilization matrices were lignocellulosic materials, sugar cane bagasse, corn leaves, corn cobs and wood shavings, obtained as agro-industrial waste.
The pH was adjusted to 5.
For the fermentation performed with glucose, the productivity reached was Results and discussion Due to heterogeneity in nature and origin of agro-industrial waste used in the experiment, and to avoid interference in the subsequent process of cell immobilization, it was necessary to establish a cleaning treatment and adapting materials. Services on Demand Article. Consultado el 10 de Julio de It has greatly contributed to the development of this research. As a result we obtained 0. Two different types of bioreactors were designed, using principles of similarity.
However, fermentations with immobilized cells also have some disadvantages, such as the difficulty to predict changes in cell growth, physiology and metabolic activity, and the presence of mass transfer limitation by diffusion. Among the most widely used immobilization matrices are hydrophilic polymer gels such as alginate, carrageenate, agarose, etc. Prior to the start of the continuous process, we performed a batch stage of 12 h.